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Evaluation of a novel dog animal model for peri-implant disease: clinical, radiographic, microbiological and histological assessment

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ARTICLE DOWNLOAD

Evaluation of a novel dog animal model for peri-implant disease: clinical, radiographic, microbiological and histological assessment

10$

Orlando Martins, João Carlos Ramos, Marta Mota, Michel Dard, Carlos Viegas, Francisco Caramelo, Célia Nogueira, Teresa Gonçalves & Isabel Poiares Baptista 

Abstract

Objective

To assess longitudinal peri-implant tissue evaluation in a plaque compromised ligature free dog model, clinically, radiographically, microbiologically and histologically.

Materials and methods

Six beagle mandibular premolars and first molars were extracted. Plaque accumulated for 16 weeks. Two implants were placed per hemi-mandible. For 17 weeks, control implants (CI) in one hemi-mandible were brushed daily; test implants (TI) in the other were not. These parameters were then assessed: clinically, probing depth (PD), bleeding-on-probing (BOP), presence of plaque (PP) and clinical attachment level (CAL); radiographically, marginal bone level; microbiologically, counts for Streptococcus spp., Fusobacterium spp., Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Prevotella intermedia and total bacterial load. At week 17, histomorphometric analysis was performed (MM-ISH (mucosal margin–implant shoulder); ISH-fBIC (implant shoulder–first bone-to-implant contact); MM-aJE (mucosal margin–apical area junctional epithelium); MM-aINF (mucosal margin–apical limit of the inflammatory infiltrate); %INF (percentage of inflammatory infiltrate)).

Results

At week 17, TI had significant increased PD, BOP, PP and CAL versus baseline. All clinical variables presented intergroup differences. There was no intergroup difference for radiographic bone loss (p > 0.05). Total bacteria, Fusobacterium spp., A. actinomycetemcomitans and P. gingivalis had intergroup differences. There was no statistically significant intergroup difference for ISH-fBIC.

Conclusions

Longitudinal microbiology evaluation detected a shift period. Final intergroup microbiological differences were the basis of W17 clinical intergroup differences, with higher values in TI. Microbiological and clinical changes detected in peri-implant tissues were compatible with onset of peri-implant disease. Despite histological inflammatory intergroup difference, no histological or radiographic intergroup bone loss was detected.

Clinical relevance

This study set-up describes a valuable method for generating “true” early peri-implant defects without mechanical trauma.

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Year 2020
Language English
Format PDF
DOI 10.1007/s00784-019-03186-3