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Comparative kinetic analysis of alginate lyase and mannanase co-produced via solid-state fermentation of cow dung supplemented with seaweed wastes by a novel Streptomyces sp. Alg-S23

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Comparative kinetic analysis of alginate lyase and mannanase co-produced via solid-state fermentation of cow dung supplemented with seaweed wastes by a novel Streptomyces sp. Alg-S23

10$

Bidyut R. Mohapatra 

Abstract

The global industrial interest in the application of biocatalysts to manufacture green and sustainable chemicals necessitates cost-effective production of microbial enzymes via screening out potent microbial isolates from different environmental niches and identifying cheap feedstocks for their propagation. In this study, the biocatalytic efficacy of a novel Streptomyces sp. Alg-S23 (GenBank accession no. MK098188) isolated from putrefying Sargassum seaweed waste was assessed for the first time for co-production of alginate lyase and mannanase via solid-state fermentation of cow dung supplemented with decomposed Sargassum as the feedstocks. The optimization of the SSF feedstock components by using a 22-factorial central composite statistical design resulted in the enhanced co-production of alginate lyase and mannanase at 15 g of cow dung and 4.5 g of decomposed Sargassum biomass. Maximal activities of extracted alginate lyase and mannanase were recorded at pH 7.5 and at temperature 50 °C and 55 °C, respectively. The co-produced alginate lyase and mannanase had strong affinity toward the substrate by displaying Km and Vmax values as 0.72 mg/mL and 10.2 U/mg-protein, and 1.83 mg/mL and 78.7 U/mg-protein, respectively. Thermal denaturation kinetic analysis of the co-produced alginate lyase and mannanase at 40-60 °C suggested that both the enzymes were thermodynamically efficient by exhibiting higher values of activation, enthalpy, and free energies of denaturation. These promising enzymatic traits highlight the novel potential of cow dung supplemented with decomposed Sargassum seaweed waste as the inexpensive feedstocks for sustainable production of alginate lyase and mannanase.

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Year 2020
Language English
Format PDF
DOI 10.1007/s13399-020-00658-5