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Characterization of genetic variation and antioxidant properties in strawberry (Fragaria × ananassa Duch.) mutant genotypes

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ARTICLE DOWNLOAD

Characterization of genetic variation and antioxidant properties in strawberry (Fragaria × ananassa Duch.) mutant genotypes

10$

Jaihyunk Ryu, Woon Ji Kim, Sang Hun Kim, Kang-Seop Lee, Han-Jig Jo, Ee-Youb Kim, Sang Hoon Kim, Si-Yong Kang, Jeong-Hee Lee & Bo-Keun Ha

Abstract

Strawberry (Fragaria × ananassa Duch.) is an important fruit in the food industry. Recently, several strawberry mutant genotypes with improved agronomic traits have been developed using gamma irradiation and somaclonal variation mutagenesis. This study was conducted to examine the genetic diversity and variation among 19 novel strawberry genotypes and 3 commercial strawberry cultivars using single nucleotide polymorphism (SNP) markers generated from genotyping-by-sequencing (GBS) analysis. In addition, the bio-functional compounds of the various strawberry genotypes were examined to determine the fruit’s potential for additional uses in the food industry. Using GBS sequencing data, a total of 506,916,648 clean data reads were generated, with 376,723,429 (74.3%) mapped reads. A set of 55,733 filtered SNPs was used to perform a phylogenetic analysis, which showed that there were clear differences among the strawberry genotypes based on their original cultivar, except for two mutant genotypes. The ellagic acid levels of 22 strawberry genotypes ranged from 1.71 to 12.10 mg/100 g with an average of 4.36 mg/100 g. Pelargonidin-3-glucoside was the major anthocyanin in 17 strawberry genotypes; however, cyanidin-3-glucoside was the major anthocyanin in two purple flower genotypes. Anthocyanin pigments were not detectable in the three white fruit genotypes. This study demonstrated that the SNPs and functional compound content are an efficient tool for mutant screening and for the selection of elite genotypes in strawberry breeding programs.

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Year 2020
Language English
Format PDF
DOI 10.1007/s10722-020-00918-3