ARTICLE DOWNLOAD

Biotransformation of cladribine by a magnetic immobilizated biocatalyst of Lactobacillus animalis

10$
ARTICLE DOWNLOAD

Biotransformation of cladribine by a magnetic immobilizated biocatalyst of Lactobacillus animalis

10$

Aldana S. Lapasset Laumann, Claudia N. Britos, Valeria A. Cappa, Cintia W. Rivero & Jorge A. Trelles 

Abstract

A stable biocatalyst with magnetic properties based on immobilized Lactobacillus animalis ATCC 35,046 to obtain 2-chloroadenine-2′-deoxyriboside, known as cladribine, is reported for the first time. This nucleoside analogue is an antitumor agent used in the treatment of a wide variety of types of leukemia. In this study, an eco-compatible and alternative bioprocess to obtain cladribine was developed. Product conversion was close to 90% at 2 h in optimized nonconventional reaction media. The microscale biosynthesis of the compound of interest afforded a total productivity close to 370 mg/L/h in the presence of DMSO, and it was stable at least for 30 days in storage conditions.

Only units of this product remain
Year 2020
Language English
Format PDF
DOI 10.1007/s10529-020-02845-w